Cisplatin adducts

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August undefined, 2024

WebMar 29, 2006 · Here we describe a new method to establish and characterize monoclonal antibodies (Mab) for structurally defined DNA adducts. The two major reaction products of cisplatin, the guanine-guanine (Pt- [GG]) and adenine-guanine (Pt- [AG]) intrastrand crosslinks are recognized by Mab R-C18 and R-B3, respectively. WebSep 24, 2024 · Cisplatin (CDDP) is the drug of choice against different types of cancer. However, tumor cells can acquire resistance to the damage caused by cisplatin, …

Cisplatin: mode of cytotoxic action and molecular basis of

WebCisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. Therefore, identifying novel biomarkers to improve the diagnosis and treatment of NSCLC is important. ... DDP binds to DNA and forms DNA-platinum adducts, which disrupt DNA synthesis and ... WebCisplatin is an effective, widely used drug for many cancers, which shows a high level and broad spectrum of antitumor activities. (1−6) It has been known that the binding between this drug molecule and the DNA molecule disables the physiologic activities of DNA and eventually induces cell apoptosis. chilled water calculation formula

Molecular mechanisms of cisplatin resistance - PubMed

WebJul 18, 2007 · One potential predictive marker is the formation of cisplatin-DNA adducts, which are formed when cisplatin reacts with the cellular DNA by binding to nucleotides. … WebDespite intense research efforts, no approved therapy is yet available. Cisplatin is a chemotherapeutic drug but induces acute tissue injury. Oleuropein (OLE) is a major … WebThese covalent adducts are formed by linking the 3, 4 ( pyrone) or 4', 5’ ( furan) edge of psoralen to the 5, 6 double bond of thymine. Psoralens can form two types of monoadducts and one diadduct (an interstrand crosslink) with thymine. [10] These adducts result in local distortions to DNA at the site of intercalation. chilled water air handlers

Cisplatin induced Mitochondrial DNA Damage In Dorsal Root …

Detection and Characterization of Single Cisplatin Adducts on …

WebCisplatin is a major anticancer drug that kills cancer cells by damaging their DNA. Cancer cells cope with the drug by removal of the damages with nucleotide excision repair. We … WebTherapeutic efficacy of cisplatin-based treatment of late stage urothelial carcinoma (UC) is limited by chemoresistance. To elucidate underlying mechanisms and to develop new approaches for overcoming resistance, we generated long-term cisplatin treated (LTT) UC cell lines, characterised their cisplatin response, and determined the expression of … chilled water cassetteWebThe cytotoxicity of platinum compounds is thought to be determined primarily by their DNA adducts. Cisplatin and oxaliplatin are structurally distinct, but form the same types of … chilled water air handler units

"WebIn general, combination of cisplatin with an antimetabolite, taxane, or topoisomerase inhibitor, can result in a modulation of platinum pharmacology on the DNA, for example, enhanced retention of the platinum-DNA adducts. These interactions are mostly sequence and cell type dependent. " - Cisplatin adducts

Cisplatin adducts

Neil3-mediated proteasomal degradation facilitates the repair of ...

WebApr 9, 1999 · Cisplatin is well known as a DNA-damaging agent, and the specific adducts produced in DNA have been well characterized. However, the mechanism by which … WebThe cytotoxicity of platinum compounds is thought to be determined primarily by their DNA adducts. Cisplatin and oxaliplatin are structurally distinct, but form the same types of adducts at the same sites on DNA. However, the DNA adducts are differentially recognized by a number of cellular proteins …

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WebOct 23, 2003 · Cisplatin is a neutral inorganic, square planar complex that reacts with DNA to induce its characteristic biological effects, which culminate in either repair of the DNA damage and cell survival... WebCisplatin is considered to be the most active platinum agent drug. Neoadjuvant (or induction) chemotherapy can be used for cervical cancer patients with locally advanced disease; this treatment is followed by radical surgery and/or radiation therapy. Cisplatin is considered to be the most active platinum agent drug

WebSep 5, 2011 · The recognition of inter- and intra-strand DNA adducts and the consequent generation of an apoptotic signal is often impaired in cisplatin-resistant cancer cells … WebFeb 28, 2024 · One of the main problems in chemotherapy using platinum drugs as anticancer agents is the resistance phenomenon. Synthesizing and evaluating valid alternative compounds is challenging. This review focuses on the last two years of progress in the studies of platinum (II)- and platinum (IV)-based anticancer complexes. In …

WebIn addition, defects in the mismatch-repair proteins hMSH6 and hMLH1 led to increased replicative bypass of cisplatin adducts, but not of oxaliplatin adducts. Finally, primer extension assays performed in the presence of HMG1, which is known to recognize cisplatin-damaged DNA, revealed that inhibition of translesion synthesis by HMG1 also ... WebCisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent that contains …

WebJul 1, 2008 · Although oxaliplatin and cisplatin form similar adducts on DNA, cisplatin is a poor substrate for OCT1 and OCT2 compared with oxaliplatin and is not active against colorectal cancer. These properties suggest that OCT-mediated uptake of oxaliplatin into cancer cells is a major determinant of its activity.

grace farrar cole elementary school norwellWebJan 5, 2024 · Platinum-based compounds are highly potent anti-tumor drugs in a variety of cancer types, acting through the formation of covalent cisplatin-DNA adducts 3, 4, 5. These compounds exhibit only... grace farrell facebookWebChromatin will also contain drug-adducts on proteins and these will not be recognized at all. Most people doing immunohistochemical techniques for DNA modifications … grace farms tnWebCisplatin is a chemotherapy medication used to treat a number of cancers. These include testicular cancer, ovarian cancer, cervical cancer, bladder cancer, head and neck … grace farms sipsWebA) The cisplatin activation process occurs by exchange of one or two of its chlorides for water molecules (monoaquated and diaquated, respectively). B) Cisplatin can form covalent bonds with... grace farm wineryWebJan 1, 2001 · Pt adducts on DNA extracted from cells that had been treated with cisplatin were less immunoreactive than DNA treated with cisplatin in vitro, but after heating the immunoreactivity increased such that 50% inhibition in the assay was caused by 2 … chilled water air handling unitWebSep 24, 2024 · Cisplatin exerts anticancer activity via multiple mechanisms. Its most acceptable mechanism involves the formation of DNA–platinum adducts by interacting with purine bases, activating several signal transduction pathways, and silencing or activating several genes which finally leads to apoptosis. grace farm wines