Thp1 cell lines atcc

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August undefined, 2024

WebMar 16, 2024 · Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an emerging pathogenic coronavirus, has been reported to cause excessive inflammation and … WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase …

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WebExample for Nucleofection™ of THP-1 cells 30 Transfection efficiency of THP-1 cells 24 hours post Nucleofection™. THP-1 cells (ATCC® TIB-202™) were transfected with … WebCell lines Human THP-1 cells were obtained from ATCC and passaged in growth medium containing RPMI-1640 (Corning), ... Cells were pelleted and washed once more using the same buffer. Pellets were resuspended in 50ul of 0.5% SDS and incubates for 5-10 minutes at 62’C. Next reactions were quenched with 145ul of water and 25ul is andre drummond injured

The In Vitro Immunomodulatory Effects Of Gold Nanoparticles …

WebApr 13, 2024 · Two independent dataset with BRCA cell lines, CCLE breast cancer cell lines (n = 69) , and BRCA cell lines ... (0.8 µg/mL) was added in the media to maintain selection. … WebTake a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium … WebApr 11, 2024 · and amphetamine-regulated transcript peptide (CARTp) is an anorexigenic neuropeptide whose receptor is undisclosed. Previously, we reported the specif… olympia 2032 austragungsort

Article - Human and Animal Health The Anti-inflammatory Effect …

WebJul 9, 2015 · THP1 (ATCC ® TIB-202™) human monocyte cell lines were cultured in Roswell Park Memorial Institute medium 1640 containing 10% (v / v) fetal bovine serum, 0.05 mM 2-mercaptoethanol, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.5 μg/mL amphotericin B (Wako Pure Chemicals, Osaka, Japan) at 37 °C, 5% CO 2, and in a humidified cell ... WebCell Proliferation Assay. Cytotoxicity against the human leukemic monocyte cell line THP-1 and the embryonic fibroblast cell line BALB/3T3 was measured using a fluorometric … olympia 2030 winterWebApr 13, 2024 · In particular, (1) as regards the MC class, 247 THP1 cells have been recorded, (2) as regards the NB class, 372 cells have been recorded, i.e. 115 CHP212 cells, 106 … is andre dawson in the hof

"WebThe THP1 human monocytic cell line was chosen as the cellular model to confirm the involvement of GAS5 in the regulation of MMP2 and MMP9 expression. Experiments were conducted at different stages of differentiation, from monocyte (THP1) to macrophages (THP1 + PMA (phorbol-12-myristate 13-acetate)). The effects of stimulation by LPS was … " - Thp1 cell lines atcc

Thp1 cell lines atcc

WebAfter exposure to PMA, the differentiated THP1 cells were treated with LPS, a bacterial endotoxin that has been used to trigger pro-inflammatory response in macrophage-like THP1 cells. 57 It has been shown that LPS can activate the secretion of pro-inflammatory cytokines, which includes IL-1β, IL-6, TNF-α and IFN-γ. 58 IL-1β is considered a key … WebHuman Thp1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > ATCC > human thp1 cell line. thp1 human monocyte cell line ATCC is a verified supplier ATCC ...

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Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at least twice the number of cells needed for the assay, because some cells will be lost throughout washes. In ADCP assays target cell number is usually the ... WebApr 30, 2024 · Human aortic endothelial cells (HAECs) were obtained from Lonza (Basel, Switzerland) and the human monocytic leukemia THP-1 cell line was purchased from …

WebCells lines, cell culture and vector transfections K562, THP-1, KG-1 and HL-60 cell lines were obtained from NCCS Pune, HL-60/MX2 cell line was obtained from ATCC (CRL 2257TM). All the cell lines were maintained in RPMI with 10% FBS and antibiotics. 293FT cells (a kind gift from Dr Amit Dutt, ACTREC) were cultured in DMEM with 10% FBS and ... WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM …

WebStefanie Luecke, Søren R. Paludan, in Advances in Virus Research, 2015. 2.4 AIM2. Transfected DNA of bacterial, viral, and mammalian origin causes formation of the … WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a temperature of 37 o C in a humidified incubator with …

WebApr 7, 2024 · In our calcium response assay, CXCL12 rapidly triggered the influx of calcium in THP1 cells, which was abolished by known CXCR4 antagonist AMD3100 or SKHS (1‰) treatment (Fig. 2 B). It suggested that SKHS contains potential CXCR4 antagonists, which was further evidenced by the inhibition of SKHS on CXCL12-induced ADAMTS4,5 …

WebThe THP-1 cell line (ATCC: TIB-202) is a human monocytic cell line derived from the peripheral blood of a one year old human male with acute monocytic leukemia (Tsuchiya … olympia 2034 austragungsortWebATCC thp1 human monocyte cell line Thp1 Human Monocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO … olympia 26bhs specsWebCell Lines › T › thp-1 Cells. thp-1 Cells. Organism Type: Human: ATCC no. TIB-202. Human monocyte derived from patient with acute monocytic leukemia. Tissues: Blood: … olympia 26 bar stool reviewsWebJan 13, 2024 · A THP-1 Cell Line-Based Exploration of Immune Responses Toward Heat-Treated BLG. Ying Deng 1,2, Coen Govers 1, Ellen ter Beest 1,2, Aalt-Jan van Dijk 3, Kasper Hettinga 4 and Harry J. Wichers 1,2*. 1 Food and Biobased Research, Wageningen University and Research, Wageningen, Netherlands. 2 Food Chemistry, Department of … olympia 2a hilversumWebDec 2, 2024 · The LAPC4 cell line was a gift from Prof. Helmut Klocker, the LNCaP-abl cell line was a gift from Prof. Myles Brown (DFCI, Boston), and the THP1 cell line was a gift from Prof. Saverio Minucci ... olympia 22 borculoWebAfter exposure to PMA, the differentiated THP1 cells were treated with LPS, a bacterial endotoxin that has been used to trigger pro-inflammatory response in macrophage-like … olympia 2 in 1 table and floor fanWebHuman hepatic adenocarcinoma cell line SK-HEP-1 (ATCC, HTB-52), human breast adenocarcinoma cell line MDA-MB-231 (ATCC, HTB-26), and human lung cancer cell line A549 (ATCC, CCL-185) were purchased from ATCC, and cultured in DMEM or RPMI contained with 10% FBS. 2.2. Generation of p62-Knockdown Cell Line. is andre champagne real champagne